Lycopene biodistribution is altered in 15, 15′-carotenoid monooxygenase knockout mice

15, 15’-carotenoid monooxygenase (CMOI) is generally recognized as the central carotenoid cleavage enzyme responsible for converting provitamin A carotenoids to vitamin A, while having little affinity for nonprovitamin A carotenoids, such as lycopene. To investigate the role of CMO I in carotenoid metabolism, ~90-d-old C57BL/6 × 129/SvJ [CMO I wild-type (WT)] and B6;129S6-Bcmo1tm1Dnp [CMO I knockout (KO)] mice were fed a high-fat, moderate vitamin A, cholesterol-containing diet supplemented with 150 mg/kg diet of β-carotene, lycopene, or placebo beadlets for 60 d (n = 12–14). CMO I KO mice fed lycopene (Lyc-KO) exhibited significant decreases in hepatic, spleen, and thymus lycopene concentrations and significant increases in prostate, seminal vesicles, testes, and brain lycopene concentrations comparedwithWT mice fed lycopene (Lyc-WT). Furthermore, in the serum and all tissues analyzed, excluding the testes, there was a significant increase in the percent lycopene cis isomers in Lyc-KOmice compared with Lyc-WT mice. CMO I KO mice fed β-carotene (βC-KO) had significantly lower hepatic vitamin A concentrations (17% of WT mice fed β-carotene [βC-WT]). Concordantly, βC-KO mice had higher serum and tissue β-carotene concentrations than βC-WT mice. In addition, phenotypically CMO I KO mice had significantly higher final body weights and CMO I KO female mice had significantly lower uterus weights than CMO I WT mice. In conclusion, CMO I KO mice fed low levels of vitamin A have altered lycopene biodistribution and isomer patterns and do not cleave β-carotene to vitamin A at appreciable levels.

Reference Information:

Lindshield, B.L., King, J.L., Wyss, A., Goralczyk, R., Lu, C., Ford, N.A., Erdman, J.W., Jr. “Lycopene biodistributin is altered in 15, 15′-carotenoid monooxygenase knockout mice”. Journal of Nutrition (2008) 138, 2367-2371.

This entry was posted on Tuesday, February 2nd, 2010. It is filed under Human Nutrition and is tagged with beta-carotene, carotenoid monooxygenase I, carotenoid monooxygenase II, carotenoids, knockout mice, lycopene, mice.